Critical Analysis Essay Example | Topics and Well Written Essays - 750 words - 6

Critical Analysis - Essay Example In this paper, the authors report on the effects of PDNF on tyrosine hydroxylase (TH) activity, a rate-limiting enzyme during the synthesis of dopamine and other catecholamine neurotransmitters. TH is important because it is involved in increasing the production of neurotransmitters. The study came up with the following additional actions of PDNF: Strengths. The paper has many strengths. First, it presented data that was based on solid experimentation that covered a wide range of immunological, molecular, and imaging techniques. The data was clearly presented using graphs and images. The discussion was straightforward and concise. The strongest point of the study is the methodology followed to arrive at the results. The authors arrived at their results through a series of experiments that each showed the action of PDNF on TH. To show that PDNF increased the TH expression in PC12 cells and primary mesencephalic neurons, the authors grew the cells in media that was treated several times with PDNF. Results obtained with immunostains were confirmed using immunoblots. Western blots and SDS-PAGE were also utilized. The study also used a luciferase gene assay to check the effect of PDNF on the promoter of TH. The method used to confirm the mechanism of action of PDNF on TH was done in a simple manner, where a construct of a mutation for the cAMP response element (CRE) labelled with luciferase transfected to PC12 cells. This step was able to show the effect of CRE on the action of PDNF on TH promoter activity. The discussion portion was able to summarize in a clear manner the results of the experiments, and related these to previous work done in the field of TH activation. The paper points to the mechanisms that could explain the action of PDNF. As an example, it was pointed out that PDNF mediated the phosphorylation of serine residues in the catalytic sites of TH. The phosphorylation activity of PDNF was

Target Market Essay Example | Topics and Well Written Essays - 500 words

Target Market - Essay Example From a layman’s point of view, the governmental and institutional sectors provide good growth opportunities. However, on a broader horizon, it can be seen that world markets other than USA provide exponential growth opportunities. These regions include Pakistan, the 2nd largest consumer of CNG vehicles where government has heavily invested in the CNG infrastructure, Bangladesh and India. Due to the increased environmental awareness amongst the target market and the technological innovations which have improved the quality and efficiency of CNG buses to match the diesel buses. Moreover, the experience of Argentina and Pakistan has shown that it is tried and tested and the investment of the investors will not sink. Moreover, it would save the huge bill on fuel and is economical. The present hike in fuel prices (petrol and diesel) is another factor which is in favor of converting fleets to CNG powered vehicles. The common observation is that even though the capital investment on a CNG bus might be US $ 25000 expensive as compared to traditional diesel buses, but the savings due to fuel economy are enough to cover up this gap in as small a time frame as 1 year. Moreover, CNG, contrary to traditional belief, has been proven to be a safe and hazard proof fuel. Considering the above now is the perfect timing to enter into this sector. Considering the above statistics, it can be concluded that Asian markets are the fastest growing markets for the CNG industry. The North and South American regions together hold immense unexploited potential and growth is expected in upcoming years. Growing global markets: the greatest opportunity for CNG Buses is the dynamic growth being experienced in the CNG sector in economies like Latin America (Argentina, Brazil), Pakistan, India, Bangladesh and Thailand. Awareness in Institutional Clients: Over a period of years, the environmental awareness has been increasing. This is